BN1 treatment resulted in 43.6% and 93.54% increases in root and shoot dry weights respectively, as compared to control. Pot trial study resulted in statistically significant increase in seedling biomass besides reduction in root rot symptoms in chir-pine seedlings. The cell-free culture filtrate of BN1 inhibited the growth of M. BN1 resulted in vacuolation, hyphal squeezing, swelling, abnormal branching and lysis of mycelia. It was characterized and selected for the present studies. An isolate, Bacillus subtilis BN1 exhibited strong antagonistic activity against Macrophomina phaseolina, and other phytopathogens including Fusarium oxysporum and Rhizoctonia solani. Use of PGPR has become a common practice in many regions of the world, and greater application of PGPR is possible for sustainable agriculture in near future.īacterial isolates having antifungal and good plant growth-promoting attributes were isolated from chir-pine (Pinus roxburghii) rhizosphere. Recent progress in our understanding of their diversity, colonizing ability, and mechanisms of action, formulation, and their application may facilitate their development as reliable biocontrol agents against plant pathogens. Although significant control of plant pathogens has been demonstrated by PGPR in laboratory and greenhouse studies, results in the field trials have been inconsistent. These rhizobacteria stimulate plant growth directly by producing growth hormones and improving nutrient uptake or indirectly by changing microbial balance in favor of beneficial microorganisms in the rhizosphere and can suppress a broad spectrum of bacterial, fungal, nematode, and even some viral diseases. PGPR can profoundly improve seed germination, root development, and water uptake by plants. Plant growth promoting rhizobacteria (PGPR) are the important group of microorganisms, which play a major role in the biocontrol of plant pathogens.

Increasing knowledge and growing concern of pesticide applications on environment have aroused interest in alternative methods of plant protection. The management of plant diseases in the sustainable agriculture has become a challenge for plant pathologist.

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